Your browser doesn't support javascript.
loading
Antioxidant effect of salvianolic acid B on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury.
Jiang, Yu-feng; Liu, Zhi-qin; Cui, Wei; Zhang, Wen-tong; Gong, Jia-pei; Wang, Xi-mei; Zhang, Ying; Yang, Mei-juan.
Affiliation
  • Jiang YF; Department of Pathology, Beijing University of Chinese Medicine, Beijing, 100029, China, jiangyf66@aliyun.com.
Chin J Integr Med ; 21(7): 516-22, 2015 Jul.
Article in En | MEDLINE | ID: mdl-25081897
OBJETIVE: To investigate the neuroprotective effects and underlying mechanisms of salvianolic acid B (Sal B) extracted from Salvia miltiorrhiza on hippocampal CA1 neurons in mice with cerebral ischemia reperfusion injury. METHODS: Forty male National Institute of Health (NIH) mice were randomly divided into 4 groups with 10 animals each, including the sham group, the model group, the SalB group (SalB 22.5 mg/kg) and the nimodipine (Nim) group (Nim 1 mg/kg). A mouse model of cerebral ischemia and reperfusion injury was established by bilateral carotid artery occlusion for 30 min followed by 24-h reperfusion. The malondialdehyde (MDA) content, the nitric oxide synthase (NOS) activity, the superoxide dismutase (SOD) activity and total antioxidant capability (T-AOC) of the pallium were determined by biochemistry methods. The morphologic changes and Bcl-2 and Bax protein expression in hippocampal CA1 neurons were observed by using hematoxylineosin staining and immunohistochemistry staining, respectively. RESULTS: In the SalB group, the MDA content and the NOS activity of the pallium in cerebral ischemia-reperfusion mice significantly decreased and the SOD activity and the T-AOC significantly increased, as compared with the model group (P<0.05 or P<0.01). The SalB treatment also rescued neuronal loss (P<0.01) in the hippocampal CA1 region, strongly promoted Bcl-2 protein expression (P<0.01) and inhibited Bax protein expression (P<0.05). CONCLUSIONS: SalB increases the level of antioxidant substances and decreases free radicals production. Moreover, it also improves Bcl-2 expression and reduces Bax expression. SalB may exert the neuroprotective effect through mitochondria-dependent pathway on hippocampal CA1 neurons in mice with cerebral ischemia and reperfusion injury and suggested that SalB represents a promising candidate for the prevention and treatment of ischemic cerebrovascular disease.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Benzofurans / Reperfusion Injury / Brain Ischemia / CA1 Region, Hippocampal / Neurons / Antioxidants Limits: Animals Language: En Journal: Chin J Integr Med Journal subject: TERAPIAS COMPLEMENTARES Year: 2015 Document type: Article Country of publication:

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Benzofurans / Reperfusion Injury / Brain Ischemia / CA1 Region, Hippocampal / Neurons / Antioxidants Limits: Animals Language: En Journal: Chin J Integr Med Journal subject: TERAPIAS COMPLEMENTARES Year: 2015 Document type: Article Country of publication: